Now that you have a nice flat image after dividing by the brightfield image, you may see that the white…
As I mentioned before, anisotropic illumination occurs when the bulb does not make the background uniformly white (or whatever color…
Image acquisition: The slide: Clean the slide. Really. When we look through the scope, we unconsciously edit out dust and…
The setup: The first thing is to acquire the best image you can before you do any postprocessing. It’s…
A couple of people have mentioned to me that they have trouble getting good looking photomicrographs. I thought I’d write…
One of the things that drew me to pathology was the aesthetics of histology. I find microscopic images fascinating and…
Here’s an example of the reconstruction of a skull from 2D photographs using the open source software “Meshroom” from AliceVision. …
Nice example of basilar vacuolization of the proximal tubules of the kidney in a case of diabetic ketoacidosis. This is…
I’ve been playing around a little with some HDR software to see if it might be useful in photomicrography. My…