Now that you have a nice flat image after dividing by the brightfield image, you may see that the white…
As I mentioned before, anisotropic illumination occurs when the bulb does not make the background uniformly white (or whatever color…
Image acquisition: The slide: Clean the slide. Really. When we look through the scope, we unconsciously edit out dust and…
The setup: The first thing is to acquire the best image you can before you do any postprocessing. It’s…
A couple of people have mentioned to me that they have trouble getting good looking photomicrographs. I thought I’d write…
A 72-year old man suffered a car crash and died. At autopsy, he was noted to have a 4 cm…