<\/p>\n
The setup:<\/strong><\/em><\/p>\n The first thing is to acquire the best image you can before you do any postprocessing.\u00a0 \u00a0It’s easier to fix (or ignore) minor problems than to fix major ones.\u00a0 So, turn the major ones into minor ones with a good setup.\u00a0 That means using a good camera, light tube, and microscope.\u00a0 I have never been all that impressed with most of the built-in cameras I’ve seen sold with microscopes, but I haven’t been keeping up with them.\u00a0 They might be fine.\u00a0 My setup is a Nikon Z7 on a 40-year-old Olympus BH-2, with a halogen bulb.\u00a0 A lot of people swear by new scopes with LED illumination.\u00a0 We have some Zeiss scopes at work like that.\u00a0 I’m not such a big fan, but that’s a matter of taste.\u00a0 Things seem a bit over-refractive to me on them.\u00a0 \u00a0I’m assuming that folk will be using a digital camera. I vaguely remember various filters and tricks for film when I was just a tyke, but I’ve forgotten it, and I don’t know anybody who uses film any more.<\/span><\/p>\n If you are building your own camera setup, you will likely have to buy a tube to put the camera on.\u00a0 Remember that these have lenses inside of them.\u00a0 If they are not good lenses, they can introduce distortion.\u00a0 I bought one for my first camera setup off of Ebay, and it was a problem.\u00a0 It worked, but it degraded the sharpness of my results no matter what I did.\u00a0 In addition, make sure that the tubes don’t magnify too much — which will limit your field of view in the image.\u00a0 For some reason I don’t understand, it seems to be hard to find a light tube that doesn’t at least give at least 2x magnification.<\/p>\n Once the system is set up, make sure that you have your focus the best it can be on the camera sensor.\u00a0 You can use the focus on the light tube to try to get the focus on the camera as close to the point of focus through the eyepieces.\u00a0 That makes things convenient, but not necessary.\u00a0 You will be focusing using a monitor attached to the camera.<\/p>\n I have the hdmi output from the camera attached to a high resolution monitor.\u00a0 I like to have the monitor at the highest resolution I can afford.\u00a0 If I’m catching a 8Kx4K image and viewing it on 1028×780 screen, then each pixel on the screen is the average of something like 40 camera pixels.\u00a0 That means you may not be able to see to focus as best you can.\u00a0 With a “4K” (3800×2480) screen, each pixel on the screen represents around 3 camera pixels. You can get around this a little by using the zoom function in your camera if you have it.\u00a0 This may not be as big a deal if you are going to scale everything down to 2Kx1K images for using on web pages and Powerpoint slides, but remember it’s always easy to degrade a good image, and hard to improve a bad one.\u00a0 It’s better to get the best image you can and degrade it for presentation on low-resolution media.<\/p>\n <\/p>\n","protected":false},"excerpt":{"rendered":" The setup: The first thing is to acquire the best image you can before you do any postprocessing.\u00a0 \u00a0It’s…<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[99,60,1],"tags":[56,27,25,26],"class_list":["post-1472","post","type-post","status-publish","format-standard","hentry","category-forensic-pathology","category-photomicrographs","category-uncategorized","tag-histology","tag-image-processing","tag-microscopy","tag-photomicrography"],"_links":{"self":[{"href":"https:\/\/wordpress.forensicpath.us\/index.php\/wp-json\/wp\/v2\/posts\/1472","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wordpress.forensicpath.us\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wordpress.forensicpath.us\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wordpress.forensicpath.us\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/wordpress.forensicpath.us\/index.php\/wp-json\/wp\/v2\/comments?post=1472"}],"version-history":[{"count":2,"href":"https:\/\/wordpress.forensicpath.us\/index.php\/wp-json\/wp\/v2\/posts\/1472\/revisions"}],"predecessor-version":[{"id":1476,"href":"https:\/\/wordpress.forensicpath.us\/index.php\/wp-json\/wp\/v2\/posts\/1472\/revisions\/1476"}],"wp:attachment":[{"href":"https:\/\/wordpress.forensicpath.us\/index.php\/wp-json\/wp\/v2\/media?parent=1472"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wordpress.forensicpath.us\/index.php\/wp-json\/wp\/v2\/categories?post=1472"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wordpress.forensicpath.us\/index.php\/wp-json\/wp\/v2\/tags?post=1472"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}