{"id":1440,"date":"2023-08-30T12:31:19","date_gmt":"2023-08-30T16:31:19","guid":{"rendered":"http:\/\/wordpress.forensicpath.us\/?p=1440"},"modified":"2023-09-26T08:34:00","modified_gmt":"2023-09-26T12:34:00","slug":"the-difference-a-camera-makes-in-photomicrography","status":"publish","type":"post","link":"https:\/\/wordpress.forensicpath.us\/index.php\/2023\/08\/30\/the-difference-a-camera-makes-in-photomicrography\/","title":{"rendered":"The difference a camera makes in photomicrography"},"content":{"rendered":"

For the first few years I did digital photomicrograpy, I used a Nikon D90.\u00a0 A couple of years ago, I bought a Nikon Z7.\u00a0 \u00a0I thought it might be interesting to compare the results using the same slide.\u00a0 Note that the light tubes are different for the D90 and Z7, so the resulting magnification is a little different.\u00a0 Thus the fields are not *exactly* the same.\u00a0 Nonetheless, the difference is pretty obvious.\u00a0 Also, the D90 takes a nominal 12 MP image (4288×2848) while the Z7 takes a 40 MP image (8256 x 5504).\u00a0 \u00a0 These are pictures of a random lung field.<\/p>\n

Here is low power.\u00a0 As you might expect, the field of view was much larger on the Z7.\u00a0 To compare the two, I put them both in a viewing app (Gwenview for linux), zoomed to the same region in both, and did a screen capture.\u00a0 it’s not a “real” image comparison with great metrics, but it gives you an idea of the visual difference.\u00a0 the D90 is on the left, the Z7 is on the right.\u00a0 You can click on the image to get a larger view.<\/p>\n

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Here’s a medium power.\u00a0 \u00a0The difference is a little more apparent.<\/p>\n

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Here is high power, where the difference is most apparent:<\/p>\n

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There could be a lot of valid criticisms to this comparison.\u00a0 Some of this is almost certainly due to the light tubes the connect that cameras to the microscope.\u00a0 They have their own lenses and magnification.\u00a0 At high power, part of the difference may be to level of focus.\u00a0 And, of course, comparing appearance on a screen is not really comparing the images.\u00a0 Nonetheless, in terms of what I can accomplish with my setup, moving to a better camera made a huge difference, particularly at high power.\u00a0 \u00a0In addition, of course, the Z7 provides NEF images, so I can play all sorts of games in creating the image.\u00a0 These are at default settings for creating jpegs.<\/p>\n

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For the first few years I did digital photomicrograpy, I used a Nikon D90.\u00a0 A couple of years ago, I…<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[99,60,1],"tags":[56,65,87,26],"class_list":["post-1440","post","type-post","status-publish","format-standard","hentry","category-forensic-pathology","category-photomicrographs","category-uncategorized","tag-histology","tag-lung","tag-photography","tag-photomicrography"],"_links":{"self":[{"href":"https:\/\/wordpress.forensicpath.us\/index.php\/wp-json\/wp\/v2\/posts\/1440","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wordpress.forensicpath.us\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wordpress.forensicpath.us\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wordpress.forensicpath.us\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/wordpress.forensicpath.us\/index.php\/wp-json\/wp\/v2\/comments?post=1440"}],"version-history":[{"count":6,"href":"https:\/\/wordpress.forensicpath.us\/index.php\/wp-json\/wp\/v2\/posts\/1440\/revisions"}],"predecessor-version":[{"id":1493,"href":"https:\/\/wordpress.forensicpath.us\/index.php\/wp-json\/wp\/v2\/posts\/1440\/revisions\/1493"}],"wp:attachment":[{"href":"https:\/\/wordpress.forensicpath.us\/index.php\/wp-json\/wp\/v2\/media?parent=1440"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wordpress.forensicpath.us\/index.php\/wp-json\/wp\/v2\/categories?post=1440"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wordpress.forensicpath.us\/index.php\/wp-json\/wp\/v2\/tags?post=1440"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}